recombinant ad-cre virus Search Results


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Bmp-mTORC1 signaling induces expression of protein anabolism genes. (A) Western blot of protein extracts from long bones at P33. Examples are shown for two mice with each genotype. (B) qRT-PCR with RNA isolated from calvaria at P33. (C) qRT-PCR with RNA isolated from Bmpr1af/f newborn calvarial cells infected with Ad-Cre or Ad-Gfp virus followed by vehicle or <t>BMP2</t> treatment for 96 h. (D) Western blot in ST2 cells following 1 h treatment with vehicle (Veh) or BMP2. Phosphorylated ribosomal protein S6 (P-S6) is normalized to S6. Quantification (average with s.d.) is shown for three independent experiments. (E) qRT-PCR with RNA from ST2 cells treated with BMP2 or vehicle for 72 h with or without chemical inhibitors. *P<0.05, n=3. WT, Bmpr1af/f; MT, Dmp1-Cre; Bmpr1af/f.
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Image Search Results


Bmp-mTORC1 signaling induces expression of protein anabolism genes. (A) Western blot of protein extracts from long bones at P33. Examples are shown for two mice with each genotype. (B) qRT-PCR with RNA isolated from calvaria at P33. (C) qRT-PCR with RNA isolated from Bmpr1af/f newborn calvarial cells infected with Ad-Cre or Ad-Gfp virus followed by vehicle or BMP2 treatment for 96 h. (D) Western blot in ST2 cells following 1 h treatment with vehicle (Veh) or BMP2. Phosphorylated ribosomal protein S6 (P-S6) is normalized to S6. Quantification (average with s.d.) is shown for three independent experiments. (E) qRT-PCR with RNA from ST2 cells treated with BMP2 or vehicle for 72 h with or without chemical inhibitors. *P<0.05, n=3. WT, Bmpr1af/f; MT, Dmp1-Cre; Bmpr1af/f.

Journal: Development (Cambridge, England)

Article Title: Dual function of Bmpr1a signaling in restricting preosteoblast proliferation and stimulating osteoblast activity in mouse

doi: 10.1242/dev.126227

Figure Lengend Snippet: Bmp-mTORC1 signaling induces expression of protein anabolism genes. (A) Western blot of protein extracts from long bones at P33. Examples are shown for two mice with each genotype. (B) qRT-PCR with RNA isolated from calvaria at P33. (C) qRT-PCR with RNA isolated from Bmpr1af/f newborn calvarial cells infected with Ad-Cre or Ad-Gfp virus followed by vehicle or BMP2 treatment for 96 h. (D) Western blot in ST2 cells following 1 h treatment with vehicle (Veh) or BMP2. Phosphorylated ribosomal protein S6 (P-S6) is normalized to S6. Quantification (average with s.d.) is shown for three independent experiments. (E) qRT-PCR with RNA from ST2 cells treated with BMP2 or vehicle for 72 h with or without chemical inhibitors. *P<0.05, n=3. WT, Bmpr1af/f; MT, Dmp1-Cre; Bmpr1af/f.

Article Snippet: For adenovirus infections, cells were incubated with Ad-Cre or Ad-Gfp virus (MOI of 100) for 6 h. At 24 h after infection, cells were split into 24-well plates and grown to confluence before being treated with vehicle (4 μM HCl) or 300 ng/ml recombinant human BMP2 (R&D Systems) for 96 h and harvested for qRT-PCR experiments.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Isolation, Infection

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: CRACD Loss Induces Neuroendocrine Cell Plasticity of Lung Adenocarcinoma

doi: 10.1016/j.celrep.2024.114286

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Ki67 (MKI67) Abcam Cat# ab16667; RRID:AB_302459 Mouse anti-Cytokeratin 19 (KRT19) Abcam Cat# ab194399; RRID:AB 2936398 Rabbit anti- Synaptophysin (SYP) Cell Signaling Cat# 36406; RRID: AB 2799098 Rabbit anti-CGRP Immunostar Cat# 24112; RRID: AB 572217 Rabbit anti-Chromogranin A (CHGA) Abcam Cat# ab15160; RRID: AB 301704 Mouse anti-Hop (HOPX) SantaCruz Cat# sc-398703; RRID: AB 2687966 Rabbit anti-Prosurfactant Protein C (SPC) Abcam Cat# ab40879; RRID: AB 777473 Rabbit anti-Uteroglobin (SCGB1A1) Abcam Cat# ab40873; RRID: AB 778766 Mouse anti-Tubulin, Acetylated (Ac-Tub) Sigma Cat# T6793; RRID: AB 477585 Mouse anti-E-cadherin Cell Signaling Cat# 14472; RRID: AB 2728770 Mouse anti-Beta Actin (ACTB) Proteintech Cat# 66009–1-Ig; RRID: AB 2687938 Rabbit anti-MASH1 (ASCL1) Abcam Cat# ab211327; RRID: AB 2924270 Goat anti-NEUROD1 Invitrogen Cat# PA5-47381; RRID: AB 2607915 Rat anti-CD31 MicroBeads, mouse Miltenyi Biotec Cat# 130-097-418; RRID: AB 2814657 Rat anti-CD326 (EPCAM) MicroBeads, mouse Miltenyi Biotec Cat# 130-105-958; RRID: AB 2936423 Rat anti-TER-119 MicroBeads, mouse Miltenyi Biotec Cat# 130-049-901; RRID: AB 2936424 Rat anti-CD45 MicroBeads, mouse Miltenyi Biotec Cat# 130-052-301; RRID: AB 2877061 Rat anti-CD31 FITC BD Biosciences Cat# 553372; RRID: AB 394818 Rat anti-CD45 APC BD Biosciences Cat# 559864; RRID: AB 398672 Rat anti-CD326 (EPCAM) PE/Cy7 BioLegend Cat# 118216; RRID: AB 1236471 Mouse anti-Vimentin BD Biosciences Cat# 550513; RRID: AB_393716 Mouse anti-N-Cadherin BD Biosciences Cat# 610921; RRID: AB_398236 Rabbit anti-CRAD (CRACD) Invitrogen Cat# PA5-61669; RRID: AB_2643037 Rabbit anti-UCHL1 Cell Signaling Cat# 13179; RRID: AB_2798141 Rabbit anti-GAPDH Cell Signaling Cat# 5174S; RRID: AB_10622025 Rat anti-CD44 BD Biosciences Cat# 550538; RRID: AB_393732 Rabbit anti-SOX2 Cell Signaling Cat# 14962; RRID: AB_2798664 Rabbit anti-OCT4 (POU5F1) Abcam Cat# ab19857; RRID: AB_445175 Rabbit anti-NANOG Novus Cat# NB100-58842; RRID: AB_877697 Bacterial and virus strains Ad-Cre BCM Gene Vector Core N/A Ad-Cre-sgLacZ BCM Gene Vector Core N/A Ad-Cre-sgCracd BCM Gene Vector Core N/A Chemicals, peptides, and recombinant proteins Fetal Bovine Serum Thermo Fisher Scientific Cat# SH3091003 Dulbecco’s modified Eagle’s medium [DMEM] Thermo Fisher Scientific Cat# MT10013CV advanced DMEM/F12 Thermo Fisher Scientific Cat# 12634010 Roswell Park Memorial Institute (RPMI) 1640 medium Thermo Fisher Scientific Cat# MT10040CV Leibovitz's L-15 Medium, no phenol red Thermo Fisher Scientific Cat# 21083027 Opti-MEM ™ I Reduced Serum Medium Thermo Fisher Scientific Cat# 31985070 0.05% trypsin-EDTA Thermo Fisher Scientific.

Techniques: Virus, Plasmid Preparation, Recombinant, Modification, Staining, Infection, Transfection, shRNA, Software